ABSTRACT
OBJECTIVE:To establish the method for content determination of cantharidin in Lytta caraganae,and to use the result as the extract screening evidence of L. caraganae source material. METHODS:Ultrasonic extraction method was used to extract cantharidin from L. caraganae using acetone as solvent. HPLC method was adopted to determine the content of cantharidin. The determination was performed on C18column with mobile phase consisted of methanol-water(23:77)at flow rate of 1.0 mL/min. The detection wavelength was set 230 nm,the column temperature was set at 35 ℃,and sample size was 10 μL. The content of cantharidin in L. caraganae was determined and compared with the results of content determination by the method stated in Chinese Pharmacopeia(using chloroform as extraction solvent). RESULTS:The liner range of cantharidin were 0.2-1.0 mg/mL (r=0.998 8)with average methodology recovery rates of 101.1%(RSD=1.7%,n=6). The average content of cantharidin in L. caraganae was 0.932%(n=3),while the content of cantharidin was 0.793%(n=3)determined by the method stated in Chinese Pharmacopeia. Both were higher than the requirement of Chinese Pharmacopeia that the content of cantharidin in scource material of cantharidin was higher than 0.35%. CONCLUSIONS:Established method is accurate and reliable for the content determination cantharidin in L. caraganae. The content of cantharidin is up to the standard of Chinese Pharmacopeia,and can be used as source material for exacting cantharidin.
ABSTRACT
Objective To observe the effect of mouse nerve growth factor (mNGF) on cognitive function and activities of daily living (ADL) in patients with delayed encephalopathy after acute carbon monoxide poisoning. Methods 35 patients with delayed encephalopathy after acute carbon monoxide poisoning were randomly divided into treatment group (n=18) and control group (n=18). Both groups accepted hyperbaric oxygen and medication, while the treatment group was injected with mNGF 20 mg a day in addition for 12 weeks. They were as-sessed with Loewenstein Occupational Therapy Cognitive Asessment (LOTCA) and modified Barthel Index (MBI) before and after treat-ment. Results The scores of LOTCA and MBI improved more in the treatment group than in the control group after treatment (P<0.05). Conclusion mNGF can obviously improve cognitive function and ADL for patients with delayed encephalopathy after acute carbon monox-ide poisoning.
ABSTRACT
@#Objective To observe the effect of mouse nerve growth factor (mNGF) on cognitive function and activities of daily living (ADL) in patients with delayed encephalopathy after acute carbon monoxide poisoning. Methods 35 patients with delayed encephalopathy after acute carbon monoxide poisoning were randomly divided into treatment group (n=18) and control group (n=18). Both groups accepted hyperbaric oxygen and medication, while the treatment group was injected with mNGF 20 mg a day in addition for 12 weeks. They were assessed with Loewenstein Occupational Therapy Cognitive Asessment (LOTCA) and modified Barthel Index (MBI) before and after treatment. Results The scores of LOTCA and MBI improved more in the treatment group than in the control group after treatment (P<0.05). Conclusion mNGF can obviously improve cognitive function and ADL for patients with delayed encephalopathy after acute carbon monoxide poisoning.